| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074380 |
| Chromosome: | chromosome 16 |
| Location: | 6828970 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672050 | (1 of 10) PF13460 - NAD(P)H-binding (NAD_binding_10) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGATATGCTCAAATCACTGGGGCATGGCGGCAAGCATGTACACGCGT |
| Internal bar code: | TCATATTGGCGCTAACCGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1355 |
| LEAP-Seq percent confirming: | 18.75 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTAACCTGTGCCTCCCG |
| Suggested primer 2: | CATTGATGCAAGACGCCGAG |