| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074385 |
| Chromosome: | plastome |
| Location: | 92031 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802302 | pafII,ycf4 | photosystem I assembly factor II; (1 of 1) PF02392 - Ycf4 (Ycf4) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATACCCTAAGTTTACTCATTCTAGGGGAAGGGGACGTCCACTGCCATCC |
| Internal bar code: | AGGGTACAGGGGCCTCCGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 5.26316 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGGGCGTCGTAAAGAAG |
| Suggested primer 2: | AACGTGCCTTTTAAGCTGCG |