| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074394 |
| Chromosome: | chromosome 6 |
| Location: | 4030100 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278215 | HIBCH1 | (1 of 1) 3.1.2.4 - 3-hydroxyisobutyryl-CoA hydrolase; 3-hydroxyisobutyryl-CoA hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCACGCGCCCGCTCTTCTGCGCACCTGTGAGCCTCTCCCCCGTCAGG |
| Internal bar code: | GCGTCCTAAACCACATCACGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTCGAATCTCGTGCTC |
| Suggested primer 2: | GCTATGCCGTCCTTACCTCC |