| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.074399 |
| Chromosome: | chromosome 12 |
| Location: | 60718 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483720 | (1 of 2) PTHR10302//PTHR10302:SF8 - SINGLE-STRANDED DNA-BINDING PROTEIN // PROTEIN OSB2, CHLOROPLASTIC-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATCCCAACATCCCCGACTTCCGTAACAAAATGGATCGCGGCAAGGCGG |
| Internal bar code: | GAGTGGTCGTAAGTCAACGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1012 |
| LEAP-Seq percent confirming: | 39.0244 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATTTCTCAGGGTTCGCCT |
| Suggested primer 2: | GCCACGTCTGTTACCCTCAA |