| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074400 |
| Chromosome: | chromosome 15 |
| Location: | 5751907 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143507 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGAGATTCGCGGACGCAACTCCAAGCCACGCACCTCTCGCCGCCCTAA |
| Internal bar code: | GCTGGACTGAAATTAGTCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGAGAGGGATTCGTCGGA |
| Suggested primer 2: | TGCCCAGTCACCACATTTGT |