Insertion junction: CLIP2.074409_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.074409
Chromosome:chromosome 2
Location:2990384
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095450 FKB16F,FKB9,FKB16-6 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 1) PTHR10516:SF145 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP42 3'UTR
Cre02.g095500 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGAAGGTGGCAGAGGATGCGGGACTCCCAACTGAACTCATGAAGTGGA
Internal bar code:TTACCGTGATGTTATTGTTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1735
LEAP-Seq percent confirming:76.0
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:75

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGCTTGAATCTGCCTGGA
Suggested primer 2:CGCATTGCTTCCTTGTGCTT