| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.074419 |
| Chromosome: | chromosome 8 |
| Location: | 3036123 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375150 | (1 of 1) PF11904//PF12796 - GPCR-chaperone (GPCR_chapero_1) // Ankyrin repeats (3 copies) (Ank_2) | 5'UTR | |
| Cre08.g375200 | (1 of 1) K07870 - Ras homolog gene family, member T1 (RHOT1, ARHT1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAACAACACCAACAGGGCAACTTGCACCGAAGCTTCGCCTGCGCGAGA |
| Internal bar code: | GCCTCCTGAGTGTGTATGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1069 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGGATGTCGGTGTTAAGC |
| Suggested primer 2: | TGGTGGACAGAGGTCAAGGA |