| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074428 |
| Chromosome: | chromosome 3 |
| Location: | 8436002 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g212977 | (1 of 1) IPR000104//IPR000197 - Antifreeze protein, type I // Zinc finger, TAZ-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCCGGTGACGGCTCGGCATGCCGCGTGTTGGCGACGCACTGGCGGAGT |
| Internal bar code: | GTTGGTACTGTGAAGTCAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGTCCTTGTACGGCATT |
| Suggested primer 2: | ACCAACACCTACATCGCAGG |