| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074454 |
| Chromosome: | chromosome 15 |
| Location: | 5672089 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre43.g760547 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGCAGTGGTGGCGGCGGCGGTGCCCTCGGCGGCGGCGGCGGCGGCGG |
| Internal bar code: | AGTTCGGCAGAACTGTTGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1064 |
| LEAP-Seq percent confirming: | 13.7931 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTTGATGCTGATGCTGC |
| Suggested primer 2: | CATCCACACTGGCCTCCATT |