Insertion junction: CLIP2.074456_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.074456
Chromosome:chromosome 7
Location:841100
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g318500 CYA8 Putative very-long-chain fatty acid condensing enzyme; (1 of 5) 2.3.1.199 - Very-long-chain 3-oxoacyl-CoA synthase / Very-long-chain beta-ketoacyl-CoA synthase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTATGTAGCGTGTGGCACGCGAGCGGGCACCTGTCGCGCCATACCGCG
Internal bar code:GTCGTCCGTTGTTCTACGTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3037
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:AGACTGAGAACCGGCCATTG
Suggested primer 2:CTGTTGCAGGCACTTTGTCC