Insertion junction: CLIP2.074461_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.074461
Chromosome:chromosome 7
Location:2749184
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre07.g331300 AGP3 ADP-glucose pyrophosphorylase large subunit; (1 of 1) PF00132//PF00483 - Bacterial transferase hexapeptide (six repeats) (Hexapep) // Nucleotidyl transferase (NTP_transferase) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCTGCTGGTGCCTCCTCTTGAGGCTGCGCGCTGCCATATCAAAGGC
Internal bar code:CTACCGGGAAGGGGCGCGAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:163
LEAP-Seq percent confirming:46.1538
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGGTATGACCCCACCACC
Suggested primer 2:CTGTTGCGGCAATCAACACA