| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074464 |
| Chromosome: | chromosome 4 |
| Location: | 379223 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217910 | (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese) | 3'UTR | |
| Cre04.g217911 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGGCTGCAAGCCGCTGGAGTAAAGCTCCGAAGCTGGGAGTAGGGAG |
| Internal bar code: | GGCTATTATACGTACCGTTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3477 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTCGTCAACTGCCGCTT |
| Suggested primer 2: | CCTTTGCCTTTGCTGTCCAC |