| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074466 |
| Chromosome: | chromosome 4 |
| Location: | 329948 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217903 | (1 of 1) IPR002711//IPR003615 - HNH endonuclease // HNH nuclease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACATTACCGCAATCCACAGACCACGAAGGTAGACTACTCAGCTAGCGTC |
| Internal bar code: | TATTAGAGTTGGGTGACAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1185 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGGAGAAGGAGTGGACTA |
| Suggested primer 2: | TACCGGTATTTGGCGTACGG |