Insertion junction: CLIP2.074468_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.074468
Chromosome:chromosome 3
Location:5604595
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g185700 3'UTR
Cre03.g185750 (1 of 1) IPR003613//IPR013083 - U box domain // Zinc finger, RING/FYVE/PHD-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGGCTGTAACGACCATAAAGCGACACCAATACTTTGACCAACTCAGT
Internal bar code:CACTGGGTAATCAAAACTACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2789
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CGTATGACGGAGGAGACGTG
Suggested primer 2:CTGTGCTGGTTTGCTGTGAC