Insertion junction: CLIP2.074473_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.074473
Chromosome:plastome
Location:178873
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802330 orf2971,ftsH,ChreCp065,2716962 ATP-dependent zinc metalloprotease; (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTAACAGTCGTCCTTTAAATCACTATAACCAAGCATTAAAATTAATTA
Internal bar code:GGGACGTCCGCTAACATCTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:87
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGATGGGAAAGCGGTTTTGG
Suggested primer 2:GGGGAAGGGGAGAGTGGTAT