| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074475 |
| Chromosome: | chromosome 14 |
| Location: | 3477239 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630550 | DEG1B,DEG13,DEG12 | Deg protease; (1 of 3) PTHR22939//PTHR22939:SF81 - SERINE PROTEASE FAMILY S1C HTRA-RELATED // PROTEASE DO-LIKE 1, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAAGGTGTGTGCGTGTGTGTGTATGTGCGTGTGCTTGTAGCCGGACG |
| Internal bar code: | TCAGGTTCTCAATTTAGCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCTTACGGTACCCTCCC |
| Suggested primer 2: | GAGCGGGTTGATACGGATGT |