| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074477 |
| Chromosome: | chromosome 16 |
| Location: | 294459 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801865 | (1 of 1) 1.14.11.42 - tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGAAGTGAAGGCGTAGCCAGGAACAGTGGTAGCGCAAACGCGTTGTTA |
| Internal bar code: | GGAGAATTCGAATAGCTGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 410 |
| LEAP-Seq percent confirming: | 62.2222 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTGGAACCCACATCACG |
| Suggested primer 2: | AGGAGGAGCAGGAGGAAGAG |