| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074480 |
| Chromosome: | chromosome 3 |
| Location: | 380822 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144727 | RBL2 | Rhomboid-like protease; (1 of 2) 3.4.21.105//3.4.21.108 - Rhomboid protease // HtrA2 peptidase / Serine proteinase OMI | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCAGCCTGCTGGTGCTGGCGCTGGTGCTGCCTCTCCAGCGCTGCGCA |
| Internal bar code: | GAATAGTTTTGTTGTGCAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 47.8261 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGACAGCTGGTGCAAAC |
| Suggested primer 2: | GTGACCCCTCCTTTTAGCCC |