| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074491 |
| Chromosome: | chromosome 7 |
| Location: | 5190500 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348850 | uL2m,MRPL2 | (1 of 1) PTHR13691:SF5 - 39S RIBOSOMAL PROTEIN L2, MITOCHONDRIAL; Mitochondrial ribosomal protein L2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCCGTGACTATTTTGACATTGCTGTTATAAGCCTACATGCCCGCTTC |
| Internal bar code: | TATATGGTACAGGCTGGTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3462 |
| LEAP-Seq percent confirming: | 98.5294 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCAGCGTTACAAGATCG |
| Suggested primer 2: | TGTGTGTGTGTTTCACCCCA |