| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.074498 |
| Chromosome: | chromosome 17 |
| Location: | 2016312 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGTGCTAACCCGCAGCACGGACTGCGCACAATCACTTAAAAATCAAG |
| Internal bar code: | AGCTTCCGCAAAGGAGGCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2731 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGGTTGTCTGCTTTGCA |
| Suggested primer 2: | CCAACGTACGTCCCTGTTCA |