| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074517 |
| Chromosome: | chromosome 17 |
| Location: | 1931876 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709950 | (1 of 1) K08272 - calcium binding protein 39 (CAB39, MO25) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAACACTCGGACAACTTGCTTCAGCAACGCCTGAGTCGGCGAGCTCAA |
| Internal bar code: | ATTAGTATACGACTAGTGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1081 |
| LEAP-Seq percent confirming: | 67.4419 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCGTTCATGCAGCGTAT |
| Suggested primer 2: | CTCGCTAGGTCTTAAGCCCG |