| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074539 |
| Chromosome: | chromosome 3 |
| Location: | 567365 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145287 | (1 of 1) PTHR24188//PTHR24188:SF39 - ANKYRIN REPEAT PROTEIN // ANKYRIN REPEAT AND SOCS BOX PROTEIN 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGGCACGGGGGTCAGCGCCCGCCGCCACCAGCTCCCGTACCAAGTCC |
| Internal bar code: | TGCATCCCATTTCTCGGCACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1926 |
| LEAP-Seq percent confirming: | 14.2857 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAACAGCTGCCTAACGGA |
| Suggested primer 2: | CGGCTCAACATCATCCCACT |