| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.074559 |
| Chromosome: | chromosome 1 |
| Location: | 2365908 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013400 | PIGS1 | (1 of 1) K05291 - phosphatidylinositol glycan, class S (PIGS); GPI transamidase component PIG-S | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCGCCGCGCACCTCCCAGGTGTGACGCGAACGCCGGCGCCGCGATCA |
| Internal bar code: | TTATTTCTTTATTAGAGTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTTGACAACTGTGCTG |
| Suggested primer 2: | TGTGTATGGGAGGGGAGGAG |