| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074563 |
| Chromosome: | chromosome 2 |
| Location: | 7303032 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391393 | (1 of 726) IPR011009 - Protein kinase-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAGTGATTGCGGTGGGGCATGCCGCCGGCAGCGATGACTGCCCCGCC |
| Internal bar code: | GTTTCATACTCACGTGGAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 5.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTAGGGAGGGGGTACAAG |
| Suggested primer 2: | TGCGCAACCCTTTGTTAAGC |