| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074566 |
| Chromosome: | chromosome 17 |
| Location: | 5027776 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735950 | (1 of 1) 4.1.1.97 - 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase / OHCU decarboxylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGATCAGATTATCCTAAGCGCCTTGACTCGTGTCACCCGTGTAGGCTT |
| Internal bar code: | AGTGCCTGCCATTCGGACCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5054 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAAAAGCACGTGCAAGCC |
| Suggested primer 2: | CTGAGTTGTTGTGGTGCACG |