| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074592 |
| Chromosome: | chromosome 3 |
| Location: | 7633926 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204400 | (1 of 1) K09651 - rhomboid domain-containing protein 1 [EC:3.4.21.-] (RHBDD1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGGCGCCAGCGGCGCCCGAGGCCGCGGCGGAATGGCACCGGCCGCTG |
| Internal bar code: | AACTTAAATCACATCGTGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 437 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGTGTAGGGAGGG |
| Suggested primer 2: | TTCGCAGTTCACGGTATGCT |