| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074617 |
| Chromosome: | chromosome 4 |
| Location: | 96822 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCACTTCAGTAGGGAGGTCCCGGGTACCCCCCCTCTCACACCTCAGT |
| Internal bar code: | TACGGTCATTTTTTTTGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 202 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTAGCAGCAGCAACAGCA |
| Suggested primer 2: | GGAGGTGTAGGTGGGATCCT |