Insertion junction: CLIP2.074647_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.074647
Chromosome:chromosome 9
Location:6391712
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g414650 (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGACGGCGCTCGGCGCTTCTAGATCAATCCCGCGACAACACCTGTCACA
Internal bar code:GTGGAGAATATATTACCGCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:6165
LEAP-Seq percent confirming:96.5909
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:88

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCATATGGCGCCGAAAC
Suggested primer 2:TCTCGATACCAACTACGCGC