| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074652 |
| Chromosome: | chromosome 8 |
| Location: | 3402593 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378000 | (1 of 1) K18749 - protein LSM14 (LSM14, RAP55, SCD6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCTGCAGTTCGGTCTTTCGGGACGGAGGGCCGGCGCGTGGGCGGCCC |
| Internal bar code: | ACAGTTTTAGTCTAACCGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 936 |
| LEAP-Seq percent confirming: | 2.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGCAGCTTCGCCAAACT |
| Suggested primer 2: | GCTTAGCAGCGCAAAATCGA |