| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074657 |
| Chromosome: | chromosome 11 |
| Location: | 3298483 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476450 | (1 of 2) PTHR22807//PTHR22807:SF34 - NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING // METHYLTRANSFERASE NSUN6-RELATED | 3'UTR | |
| Cre11.g476500 | PAO7 | Pheophorbide a oxygenase-related protein; (1 of 8) 1.14.12.20 - Pheophorbide a oxygenase / Pheide a oxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGGTTGCGCCTCGTGGGTCGTGACGTCGTGACGTTGGGTCAAAGCGG |
| Internal bar code: | ATTTGTCGCACCCTTACGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1608 |
| LEAP-Seq percent confirming: | 28.5714 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATCTTACGGAGGCAGGCC |
| Suggested primer 2: | AGATTAGAGCGAGCGTCTGC |