Insertion junction: CLIP2.074658_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.074658
Chromosome:chromosome 9
Location:2909448
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g390450 POB15 Proteome of basal body 15; (1 of 88) IPR011333 - POZ domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGATGCGACTTGCCTCGCGTAGAAGCTGGAGGGGGACGTGCAGGGCAC
Internal bar code:TCAAGTAGGGTCGCGCCACGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:325
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGAGCAAACACACACACA
Suggested primer 2:AAGGCGGTACTGATGTCGTG