Insertion junction: CLIP2.074661_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.074661
Chromosome:chromosome 6
Location:4686315
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278800 (1 of 1) PTHR11266:SF40 - MPV17/PMP22 FAMILY PROTEIN 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTACTTACACCGGCGCAACAAATATTCAAAATTAAATCAACGGCTCCA
Internal bar code:TAAATGGCTTTACCACCGTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:5393
LEAP-Seq percent confirming:80.8511
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:CACGTCAATGCTGCTGCTTT
Suggested primer 2:CGGCGGACCCAATGATATCA