| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.074662 |
| Chromosome: | chromosome 5 |
| Location: | 3607842 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCAGAAGTTCAAGGAAGCGGCCGGATTTCTTATGGTGAGGCGTGAGCT |
| Internal bar code: | CATCGTGGTTGATCATCGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 43.75 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTGCTTGGTTCAAACAT |
| Suggested primer 2: | TGCAGTCCTTGGGCTTGTAG |