| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074683 |
| Chromosome: | chromosome 9 |
| Location: | 3675412 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397179 | OPR13 | OctotricoPeptide Repeat protein 13 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAGGTATGAACTTTGTTCCGTAAAGTTGAGCCAATTCGCAATTCGCT |
| Internal bar code: | GCCGTCGGTGATAAGTAATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1903 |
| LEAP-Seq percent confirming: | 75.8621 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACACGAGGGCTCAAGTCA |
| Suggested primer 2: | AGGTGTGTGTGTGTGTGTGT |