| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074685 |
| Chromosome: | chromosome 17 |
| Location: | 3460873 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723650 | KAT1,ATO1 | (1 of 1) 2.3.1.16 - Acetyl-CoA C-acyltransferase / Beta-ketothiolase; 3-oxoacyl CoA thiolase/acetyl-CoA acyltransferase 1 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGCTTGTGTGCTTCCTAGAACACACTGCAGCTACCAAGAATACCAAC |
| Internal bar code: | CACAAACAAGTTATCTTTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3725 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTCGGATCTCTGCTCC |
| Suggested primer 2: | AGAACTGCGAGCTGTCACTC |