| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.074692 |
| Chromosome: | chromosome 11 |
| Location: | 4037735 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480650 | SDR20 | Short-chain dehydrogenase/reductase; (1 of 13) 1.1.1.300 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGGGACCGCGGCCCGCAGCCCGCAGAGCGTTGAGGCGGCAGAGTGT |
| Internal bar code: | ATTATCCTCATGTAGGCCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTAACATCGCGGCATGC |
| Suggested primer 2: | GGGTGCTTGGACCTAGGAAC |