| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074725 |
| Chromosome: | chromosome 7 |
| Location: | 3769839 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338350 | CPLD50 | Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR10072//PTHR10072:SF43 - IRON-SULFUR CLUSTER ASSEMBLY PROTEIN // SUBFAMILY NOT NAMED | 5'UTR |
| Cre07.g338400 | (1 of 1) K14845 - RAT1-interacting protein (RAI1, DOM3Z) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGCGCAGAAGTGACGCGGGTTGTTGCAAAGTTGCTTCGCGCGAGGAC |
| Internal bar code: | GAGCCCGTCCCAAGAAACATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1078 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAACCCCGTATGATGCT |
| Suggested primer 2: | CACCAGCTCTACAGGGTTGG |