Insertion junction: CLIP2.074727_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.074727
Chromosome:chromosome 8
Location:1978527
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g368350 gene_edge/mRNA_edge/5'UTR
Cre08.g368400 TR1,NTR1,TR (1 of 1) PTHR22912//PTHR22912:SF23 - DISULFIDE OXIDOREDUCTASE // THIOREDOXIN REDUCTASE 1; NADPH-dependent thioredoxin reductase 1, selenoprotein 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCCCATGCATCCCATGCCGAAAAAGGAGGACTCGAATAGGGTGCAAG
Internal bar code:TTGATTGAACCGCTATTTGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1746
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGCTTCTTGGGGATGCAG
Suggested primer 2:CAGCACTGTCCTGCTCATGA