| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074733 |
| Chromosome: | chromosome 4 |
| Location: | 1594561 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213250 | (1 of 2) K01256 - aminopeptidase N (pepN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCGGCGGCTGGGTGGGTCTCTTTCCGTTCTGTTGTTGAACACACGCC |
| Internal bar code: | CACTGCTGGGCATAAGCCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 798 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTCCCAATAGCCACCTG |
| Suggested primer 2: | ATCGCACCCCAGATGGAATC |