| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074739 |
| Chromosome: | chromosome 12 |
| Location: | 1362171 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489050 | PCT2,CDS2 | Phosphatidate cytidylyltransferase; (1 of 1) PTHR13773:SF3 - PHOSPHATIDATE CYTIDYLYLTRANSFERASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATTCTATGTTACTTGGACTACCTAGTAAACGATGGTATAACAACCAAA |
| Internal bar code: | CTCTTGTGTTTTCGGATTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3146 |
| LEAP-Seq percent confirming: | 93.0233 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAAATGACAACGGCACCG |
| Suggested primer 2: | AGTTACACACTTGCGGCGTA |