| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074741 |
| Chromosome: | chromosome 12 |
| Location: | 2671485 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505300 | (1 of 1) PF08317 - Spc7 kinetochore protein (Spc7) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACACACAAAGGTTTTGCCGTGCGGGCCTGCCCGACCAGAGCCAAGGCG |
| Internal bar code: | AGTGGGTGCGTAGCACCTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5847 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTCCCGTTTGTCCCCTA |
| Suggested primer 2: | TGTTGTGTCCGTAAGCCTCC |