| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074743 |
| Chromosome: | chromosome 7 |
| Location: | 4645117 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344600 | PGD1,PGDH1 | (1 of 1) PTHR10996:SF159 - D-3-PHOSPHOGLYCERATE DEHYDROGENASE; D-3-phosphoglycerate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTAAAAATGTTGCACTTGACAAATTACAGGGTCTGCTCTCGCTTCGTG |
| Internal bar code: | GTGAGAGGCCAGTGTACACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1506 |
| LEAP-Seq percent confirming: | 90.2439 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGGCGTTAAATTGAGGG |
| Suggested primer 2: | TGATTCCAGACCCCAACTGC |