| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.074758 |
| Chromosome: | chromosome 4 |
| Location: | 234827 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217350 | KUP4 | (1 of 5) K03549 - KUP system potassium uptake protein (kup); Potassium ion uptake transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGCCTCACCACTGACGTGCACGCGGCCGAACGCACGGATGTGGATG |
| Internal bar code: | GTGGGCATGTTTTGTGTAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 68 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCATGATGCACTGCGTG |
| Suggested primer 2: | ACGCTGTTGTATGATGGCCA |