Insertion junction: CLIP2.074777_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.074777
Chromosome:chromosome 1
Location:50541
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g000200 (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain; Esterase, SGNH hydrolase-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGGGCCCCGGTAAACTCCGAGACCAGGAGAAGATGAGAAGGCCGTGTT
Internal bar code:GTAGCGCCGGGCTTGCTCGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1979
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:GTAATGGTGCGCGGAATCAC
Suggested primer 2:GTGTTTGACAAGCTGGCAGG