| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.074803 |
| Chromosome: | chromosome 1 |
| Location: | 3003172 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018850 | EEP2 | (1 of 4) K12603 - CCR4-NOT transcription complex subunit 6 (CNOT6, CCR4); Exonuclease-Endonuclease-Phosphatase superfamily protein | 5'UTR |
| Cre01.g018900 | (1 of 1) K11971 - E3 ubiquitin-protein ligase RNF14 [EC:6.3.2.19] (RNF14, ARA54) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAGCGCACCGGTTTGGAGTAATGACACTCGTGCCTGGGCCCCTCACGC |
| Internal bar code: | TTAGGTCTTTTGCGTGGTGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2770 |
| LEAP-Seq percent confirming: | 95.3488 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGGTAACAAACGTCACAC |
| Suggested primer 2: | CCAAAGGAGGGGCCAGTAAG |