| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.074804 |
| Chromosome: | chromosome 12 |
| Location: | 1824405 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512500 | (1 of 1) PF07962 - Replication Fork Protection Component Swi3 (Swi3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCATGAGGCCATGAGCCCTCTTCGTCGCCGGGGGGCAATGCCGCTCC |
| Internal bar code: | CTTAGCCGAATTTTTACCGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1554 |
| LEAP-Seq percent confirming: | 96.4912 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATGCTGCTCAACGAGGA |
| Suggested primer 2: | AATGCCGAGTTCTCGCTACC |