Insertion junction: CLIP2.074804_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.074804
Chromosome:chromosome 12
Location:1824407
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g512500 (1 of 1) PF07962 - Replication Fork Protection Component Swi3 (Swi3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACGCCCTGGACTGAGCAGGCACCACAGCAAGATACAGCTCCAGGTCC
Internal bar code:CGTCAAAGTTGCGTGATCCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2156
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AATGCCGAGTTCTCGCTACC
Suggested primer 2:AACATGCTGCTCAACGAGGA