| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074808 |
| Chromosome: | chromosome 7 |
| Location: | 2629471 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330350 | OPR115 | OctotricoPeptide Repeat protein 115 | outside_mRNA |
| Cre07.g330400 | KCN1 | (1 of 1) K04886 - potassium voltage-gated channel Shab-related subfamily B member 2 (KCNB2); Six-transmembrane domain potassium channel alpha subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGTCATGCAAAACACGCGAGCCCCGTAAAAGACTGCATCCATCGATC |
| Internal bar code: | TTCCATCAGTCGTGGATCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5623 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACCGAAACGAGTGTCCA |
| Suggested primer 2: | GGTCGAGTTCGGTCCCTTTT |