| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074810 |
| Chromosome: | chromosome 6 |
| Location: | 3455312 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278105 | (1 of 24) IPR006311 - Twin-arginine translocation pathway, signal sequence | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAACCACCCCTAATCCCCACATCTACCGCCGCCAACTACCGTATTCAA |
| Internal bar code: | CCTGATGCGATCAGGACATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 60.8696 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTCCAATGTCCTGCTCC |
| Suggested primer 2: | GGCGGTTGTTGATGATGAGC |