| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074827 |
| Chromosome: | chromosome 5 |
| Location: | 485257 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244050 | Chlamydomonas specific protein | 3'UTR | |
| Cre05.g244100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTACTGCACATGTGGATGAGCGGGTCAAGTCCTCGCTCTGCAAATTG |
| Internal bar code: | ACTACCTTGAGGGGGATTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2796 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGACCAAGTCTGCTGTCG |
| Suggested primer 2: | ACCACGTGTTTCGGTGAGTT |